Vitrification-based Cryopreservation of Brain Slices

Experimental Overview:
This study applied VitriTech™ vitrification technology to preserve fresh brain slices, achieving successful cryopreservation for over 200 days. Upon thawing, the preserved tissues exhibited functional similarity to fresh controls in key metrics including stimulus response and action potential frequency, indicating that neuronal viability and intact electrophysiological functions were effectively preserved. This achievement represents a breakthrough in the long-term functional preservation of brain slices.

                                
A. Electrophysiological Signal Integrity

• Post-thaw brain slices maintained stable electrophysiological signals, consistent with fresh tissues. Moreover, learning and memory functions were effectively preserved.

B. Action Potential Frequency and Amplitude

• No significant differences were observed in action potential frequency or amplitude between vitrified and fresh tissues.

C. Neuronal Activity in Cortex and Hippocampus

• Vitrified tissues displayed normal neuronal activity in the cortex and hippocampus:
  ➢ Sodium/potassium currents
  ➢ Evoked potential frequency
  ➢ Membrane capacitance and resistance
  These results confirm the preservation of the brain's functional integrity post-vitrification.

Conclusion:
This pioneering study establishes a high-precision, low-damage cryopreservation protocol for brain slices, ensuring long-term preservation of electrophysiological functionality. This result provides an innovative solution for neuroscience research and neuropharmacological development, with significant translational potential in clinical applications.